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bacterial expression vector petm11 embl  (New England Biolabs)


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    Structured Review

    New England Biolabs bacterial expression vector petm11 embl
    Bacterial Expression Vector Petm11 Embl, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 33745 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bacterial+expression+vector+petm11+embl/T4+DNA+Ligase/pm34895045-87-16-28
    Average 99 stars, based on 33745 article reviews
    bacterial expression vector petm11 embl - by Bioz Stars, 2026-10
    99/100 stars

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    Expressing:

    Article Title: Small molecule inhibitor of Igf2bp1 represses Kras and a pro-oncogenic phenotype in cancer cells
    Article Snippet: Human Igf2bp1 di-domains RRM12 (M1-P188) and KH34 (P387-A574) cDNA sequences were PCR-amplified from a pCMV6-Entry vector containing the full-length sequence (Origene – NCBI reference sequence NM_006546.4), using the KOD Hot Start Master Mix (Novagen) and primers incorporating NcoI/XhoI restriction sites. .. After 1–3 h digestion at 37°C with NcoI/XhoI (NEB) the cDNA sequences were ligated into the bacterial expression vector pETM11 (EMBL) overnight at 16°C using T4 DNA ligase (NEB) (insert-to-plasmid ratios of 4:1 and 3:1 for RRM12 and KH34 respectively). .. Plasmids were amplified in DH5α C2987 E. coli (NEB) and successful cloning was confirmed by sequencing (Source Bioscience).

    Article Title: Small molecule inhibitor of Igf2bp1 represses Kras and a pro-oncogenic phenotype in cancer cells.
    Article Snippet: Human Igf2bp1 di-domains RRM12 (M1-P188) and KH34 (P387-A574) cDNA sequences were PCR-amplified from a pCMV6-Entry vector containing the full-length sequence (Origene – NCBI reference sequence NM_006546.4), using the KOD Hot Start Master Mix (Novagen) and primers incorporating NcoI/XhoI restriction sites. .. After 1–3 h digestion at 37°C with NcoI/XhoI (NEB) the cDNA sequences were ligated into the bacterial expression vector pETM11 (EMBL) overnight at 16°C using T4 DNA ligase (NEB) (insert-to-plasmid ratios of 4:1 and 3:1 for RRM12 and KH34 respectively). .. Plasmids were amplified in DH5α C2987 E. coli (NEB) and successful cloning was confirmed by sequencing (Source Bioscience).

    Plasmid Preparation:

    Article Title: Small molecule inhibitor of Igf2bp1 represses Kras and a pro-oncogenic phenotype in cancer cells
    Article Snippet: Human Igf2bp1 di-domains RRM12 (M1-P188) and KH34 (P387-A574) cDNA sequences were PCR-amplified from a pCMV6-Entry vector containing the full-length sequence (Origene – NCBI reference sequence NM_006546.4), using the KOD Hot Start Master Mix (Novagen) and primers incorporating NcoI/XhoI restriction sites. .. After 1–3 h digestion at 37°C with NcoI/XhoI (NEB) the cDNA sequences were ligated into the bacterial expression vector pETM11 (EMBL) overnight at 16°C using T4 DNA ligase (NEB) (insert-to-plasmid ratios of 4:1 and 3:1 for RRM12 and KH34 respectively). .. Plasmids were amplified in DH5α C2987 E. coli (NEB) and successful cloning was confirmed by sequencing (Source Bioscience).

    Article Title: Small molecule inhibitor of Igf2bp1 represses Kras and a pro-oncogenic phenotype in cancer cells.
    Article Snippet: Human Igf2bp1 di-domains RRM12 (M1-P188) and KH34 (P387-A574) cDNA sequences were PCR-amplified from a pCMV6-Entry vector containing the full-length sequence (Origene – NCBI reference sequence NM_006546.4), using the KOD Hot Start Master Mix (Novagen) and primers incorporating NcoI/XhoI restriction sites. .. After 1–3 h digestion at 37°C with NcoI/XhoI (NEB) the cDNA sequences were ligated into the bacterial expression vector pETM11 (EMBL) overnight at 16°C using T4 DNA ligase (NEB) (insert-to-plasmid ratios of 4:1 and 3:1 for RRM12 and KH34 respectively). .. Plasmids were amplified in DH5α C2987 E. coli (NEB) and successful cloning was confirmed by sequencing (Source Bioscience).



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    New England Biolabs bacterial expression vector petm11 embl
    Bacterial Expression Vector Petm11 Embl, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bacterial+expression+vector+petm11+embl/T4+DNA+Ligase/pm34895045-87-16-28
    Average 99 stars, based on 1 article reviews
    bacterial expression vector petm11 embl - by Bioz Stars, 2026-10
    99/100 stars
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